The FastPCR software was developed to be an integrated tools environment that provides comprehensive facilities for designing primers for most PCR applications including standard, long distance, inverse, real-time, multiplex, unique and group-specific; overlap extension PCR (OE-PCR) multi-fragments assembling cloning; single primer PCR (design of PCR primers from close located inverted repeat), automatically SSR loci detection and direct PCR primers design, amino acid sequence degenerate PCR, Polymerase Chain Assembly (PCA) or oligos assembly and much more.
The “in silico” (virtual) PCR primers or probe searching, comprehensive pairs and individual primers analysis tests are included. The “in silico” oligonucleotide search is helpful for discovering target binding sites with the temperature melting calculation.
FastPCR has the capacity to handle long sequences and sets of nucleic acid or protein sequences and it allowed the individual task and parameters for each given sequences and joining several different tasks for single run. It also allows sequence editing and databases analysis. Efficient and complete detection of various types of repeats developed and applied to the program with a visualisation.
The program includes various bioinformatics tools for analysis of sequences with GC or AT skew, CG content and purine-pyrimidine skew, the linguistic sequence complexity; generation random DNA sequence, restriction analysis and supports the clustering of sequences and consensus sequence generation and sequences similarity and conservancy analysis.
FastPCR is a software package to aid molecular biologist, students, and other in the study, manipulation and design experiment.
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FastPCR is designed to be a simple to use and yet effective PCR primer design software with a powerful data base searching and display system.
The FastPCR allows you to design nearly any primers you want, such as:
− standard PCR primers (FP and RP),
− long distance PCR primers (LD),
− inverse PCR primers (IK),
− real-time PCR primers (RT),
− multiplex PCR primers,
− single primer PCR primers (SP),
− automatic cloning primers design and OE-PCR
− direct PCR primers design
− primers dimers detection
− SSR locus detection and direct PCR primers design and much more
Since FastPCR is free edition at now, we plan to bring the PRO version at a later date, including the following:
1. Addition of a 1D Chromatogram View.
2. Addition of 3D chromatogram viewer.
3. Addition of Exclude symbol 1D and 3D chromatogram viewer.
4. Addition of standard and modern-designed primers.
5. Addition of computer-generated dissociation curves and marker details.
6. Addition of an interactive step and codon design.
7. Addition of a primer design helper and automatic primer composition.
8. Addition of a sorting and exporting system for special genes.
9. Addition of a specialized primer design for tRNA, 5S rRNA, and 5S rDNA.
10. Addition of a sequence aligning function using default parameters for selected files.
11. Addition of a consensus sequence viewer for selected sequences.
12. Addition of program editor for stand alone programs.
What Is “FastPCR Professional” Software?
FastPCR Professional software was developed to be a full contained version of FastPCR software for all the needs of molecular biologists, students, and other in bioinformatics studies and experiments. It has a lot of unique features not found in the standard FastPCR Free Edition, of an all in one software package that includes the following:
1. 1D Chromatogram View:
It is the design of a single PCR primer for a single loci. The 1D chromatogram viewer in FastPCR Professional software is the best way to show a single PCR primer design. We have improved the output formats of Fast
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FastPCR can be run on Personal computers. FastPCR allows you to design primers with FastPCR Professional, view the design, modify, and see the calculated results of the entire primer set.
It can generate a set of primers from your sequences that are around 1-2 kilobases in length. You will not have to worry about flanking sequence or sequences similar to another primer sets.
You can insert multiple sites or motifs into primer you are designing.
FastPCR generates sets of primers for you with nearly 40 different variables. These variables include GC skew, AT skew, CG skew, purine-pyrimidine skew, the base content and total sequence complexity.
When you design a primer set, FastPCR generates “close-by” primer groups, which can be amplified with one set of primers. You can also generate specific groups of primers for cloning.
FastPCR can work with DNA, RNA, cDNA, and protein sequences. You can sequence edit your genes, find sequences that are similar to your sequence or a sequence on the web.
FastPCR can also generate random sequences for you, in fact you can design random sequences of a specified size and AT/GC content.
You can specify the number of mismatches allowed in the primer when you design primers for cloning or gene amplification. FastPCR can also determine primer sequences that cannot create extension products even if they match perfectly.
FastPCR can also scan your sequence for microsatellites, which are strings of repeating elements that increase in length at regular intervals. Repeat elements occur at a variety of locations in a genome.
FastPCR can be used for making duplicate sets of primers that have degenerate primers, universal primers, and reverse primers.
If your sequence has a gap, you can specify which primers to leave out of the set when you generate the primers. FastPCR can also locate and identify primer binding sites.
FastPCR can also design primers that can be used in OE-PCR experiments
FastPCR designs primers in any direction around your sequence (just like a compass) so that they can pick up any loop that might occur. This is especially useful for designing long DNA fragments because they may have internal loops.
FastPCR can generate groups of primers. These groups can be very useful when planning cloning experiments where you might want to use a group of primers to amplify
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FastPCR is a software designed to assist molecular biologists in the exploration of their experimental data. FastPCR combines a set of analysis programs that assist in a variety of tasks. Some of the functions of the FastPCR programs include:
Combine the fastPCR Free license, unlimited use or not (3D) for free with the FastPCR Professional license for a tenfold price advantage.
FastPCR Professional has the following features:
Allows developers and researchers to do everything with limited speed but a beautiful look.
Contains a large number of tools such as “in silico” oligos search, primer design, PCR fragment overlapping, sequences similarity, assemblage cloning, direct PCR primers design (from inverted repeats), simple PCR, oligos assembly, amino acid sequence degenerate PCR, direct PCR, Polymerase Chain Assembly (PCA) or oligos assembling, amino acid sequence analysis (degenerate and non-degenerate), RNAse T1 accessibility, multiple sequence alignment (clustering, alignments and trees), multiple sequence alignment (clustering and trees), GC and AT skew, purine-pyrimidine skew, UTR analysis, GC content, CG content, NUCI analysis, direct sequences alignment, genetic code symbols codon usage, and some more.
Provides a large number of input and output file formats including: TXT, HTM, MTS, EML, PED, PML, FASTA, FASTAL, FASTA, CAST, OAS, BABEL, BWT, FASTQ, SAM, SORTED, BED, GZ and the function to convert these formats to any other file format such as: SHEX, LISP, XML, HTML, DOC, XLS, XLS, ASCII, EXCEL, HTML, PDF, SAV, ZIP, RTF, RDS, MODE, RXT, TEL, MHT, LISP, CUC, XML, GFF, FH, ZIP, BISQ, GZ, INI, HL1, LISP, POS, and many more.
Download FastPCR from our website for free
FastPCR is a free software that is downloaded directly from the Internet from our web page.
The source code of the software is available to read and modify
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Processor: Intel Core i5-2400 or AMD equivalent
Memory: 8GB RAM
Graphics: 2GB video card
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Windows 10, 64-bit: The Vulkan API requires 64-bit Windows 10 as a minimum.
Vulkan is implemented as an extension for DirectX 11 and DirectX 12. If Vulkan is installed, it is a requirement to have DirectX 11 or 12 installed. If
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