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Docosahexaenoic acid (DHA) is an essential omega-3 polyunsaturated fatty acid (PUFA) with a potential role in regulating glucose metabolism. Glucagon-like peptide-1 (GLP-1), an insulinotropic hormone, is secreted from intestinal L cells and stimulates insulin secretion and glucose-dependent pancreatic β-cell growth. GLP-1 also increases fat utilization by decreasing lipogenesis and increasing fat oxidation and energy expenditure. In the present study, we investigated how DHA intake affects the concentrations of plasma total GLP-1, amino acids, and gut microbiota-associated metabolites (for example, short-chain fatty acids [SCFAs] and trimethylamine N-oxide [TMAO]). Rats were fed diets containing DHA, docosapentaenoic acid (DPA), or fish oil (FO) for 26 wk. At the end of the experiment, plasma total GLP-1, the concentrations of amino acids and their metabolites, SCFAs, and TMAO were analyzed. Plasma total GLP-1 was increased by both DHA and DPA compared with the FO-fed rats. The DHA and DPA diets increased the concentrations of phenylalanine, aspartic acid, alanine, and lysine in plasma. In a complementary study, rats were fed for 2 wk the DHA diet, the DPA diet, or no dietary fat (control) and TMAO and SCFAs were measured at the end of the experiment. In line with our observations in the long-term experiment, DHA diet increased plasma TMAO concentrations. Additionally, plasma concentration of hippurate was increased by the D

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